DATASHEET
Host:
Mouse
Target Protein:
ICAM1/CD54
Immunogen Range:
321-420/532
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3383
Swiss Prot:
P05362
Source:
Recombinant human ICAM1/CD54
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].
VALIDATION IMAGES
HUVEC lysates probed with ICAM1/CD54 Polyclonal Antibody, Unconjugated (bs-10697M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ICAM1/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.