Host: Mouse
Target Protein: AFP
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 174
Swiss Prot: P02771
Source: Full length human AFP protein
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 69
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).Read more>>
- Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378.Read more>>
- Liu, X., et al. “Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein.” Biosensors and Bioelectronics (2017); 91:431-435.Read more>>
- Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Read more>>
- A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14Read more>>
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Lane 1: Rat Brain lysates; Lane 2: Rat Kidney lysates; Probed with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200, overnight at 4˚C. Followed secondary antibody (bs-0296G-HRP) at 1:3000 for 90min at RT.
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Rat Embryo Lysates; Lane 2: Rat Placenta Lysates. Probed with AFP(A2) monoclonal Antibody, unconjugated (bsm-1621M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.