bsm-1621M-PerCP-Cy7 [Conjugated Primary Antibody]
AFP Monoclonal Antibody, PerCP-Cy7 Conjugated
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Host: Mouse

Target Protein: AFP

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 174

Swiss Prot: P02771

Source: Full length human AFP protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M PBS (pH7.4) with 0.02% Proclin300.

Storage: Store at -20°C for 12 months.

Background:

Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Conjugation: PerCP-Cy7

Excitation/ Emission: 482nm/780nm

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:500-1000)
ELISA(1:200-400)
IHC-P(1:50-200)
IHC-F(1:50-200)
IF

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Changrong, et al. "Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition." Analytica Chimica Acta (2014).Read more>>
  • Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.Read more>>
  • Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014).Read more>>
  • Liu, Yingshuai, et al. "3-D Microarray and Its Microfabrication-Free Fluidic Immunoassay Device." Analytica Chimica Acta (2015).Read more>>
  • Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).Read more>>
  • Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378.Read more>>
  • Liu, X., et al. “Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein.” Biosensors and Bioelectronics (2017); 91:431-435.Read more>>
  • Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Read more>>
  • A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14Read more>>
  • Uma M. Nori. et al. Antibody Immobilization on Sulfated Cellulose Nanocrystals. BIOMACROMOLECULES. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES