bsm-1622M-BF750 [Conjugated Primary Antibody]
AFP (A4) Monoclonal Antibody, AbBy Fluor® 750 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: AFP

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 174

Swiss Prot: P02771

Source: Full-length AFP protein

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Conjugation: AbBy Fluor® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.Read more>>
  • Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014).Read more>>
  • Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).Read more>>
  • Liu, X., et al. “Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein.” Biosensors and Bioelectronics (2017); 91:431-435.Read more>>
  • Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019.Read more>>
  • Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Read more>>
VALIDATION IMAGES