bsm-1625M [Primary Antibody]
PSA(Pa3) Monoclonal Antibody
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DATASHEET

Host: Mouse

Target Protein: PSA

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 354

Source: Full length native protein purified from human

Purification: Purified by Protein G.

Storage Buffer: 0.01M PBS (pH7.4) with 0.02% Proclin300.

Storage: Store at -20°C for 12 months.

Background:

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:100-1000)
ELISA(1:100-1000)
IHC-P(1:100-1000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou, Jie, et al. "Spectrum-based and color-selective electrochemiluminescence immunoassay for determining human prostate specific antigen in near-infrared region." Talanta 165 (2017): 117-121.Read more>>
  • Liu, Yingshuai, et al. "A concentration− dependent multicolor conversion strategy for ultrasensitive colorimetric immunoassay with the naked eye." Analytica Chimica Acta (2017).Read more>>
  • Liu, Yingshuai, Lingli Lei, and Zeying Zhang. "An ultrasensitive colorimetric immunoassay based on glucose oxidase catalyzed cascade formation of blue–black iodine–starch complex." Sensors and Actuators B: Chemical (2017).Read more>>
  • Fanchun Zeng. et al. Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/_-catenin pathway. GENES DIS. 2022 JulRead more>>
  • Uma M. Nori. et al. Antibody Immobilization on Sulfated Cellulose Nanocrystals. BIOMACROMOLECULES. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat submandibular gland labeled with Mouse Anti-PSA(Pa3) Monoclonal Antibody (bsm-1625M) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSA(Pa3) Monoclonal Antibody, Unconjugated (bsm-1625M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSA(Pa3) Monoclonal Antibody, Unconjugated (bsm-1625M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.