bsm-2346M [Primary Antibody]
P53(wt-p53) (D2F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: P53wt-p53

Immunogen Range: 341-393/393


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7157

Source: KLH conjugated synthetic peptide derived from human P53(wt-p53)

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Juan, et al. "Sterigmatocystin induces G1 arrest in primary human esophageal epithelial cells but induces G2 arrest in immortalized cells: key mechanistic differences in these two models." Archives of Toxicology (2014): 1-11.Read more>>
  • Zhou et al. miR612 is associated with esophageal squamous cell carcinoma development and metastasis, mediated through TP53. (2017) Mol.Med.Re. 16:1855-1863Read more>>
  • Zixin Zhou. et al. CX3CR1hi macrophages sustain metabolic adaptation by relieving adipose-derived stem cell senescence in visceral adipose tissue. CELL REP. 2023 May;42:112424Read more>>
VALIDATION IMAGES

Lane 1: Mouse Esophagus cell lysates; Lane 2: Mouse Bladder cell lysates; Lane 3: Mouse Ear cell lysates probed with P53(wt-p53) (D2F7) Monoclonal Antibody, Unconjugated (bsm-2346M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.