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Host: Mouse
Target Protein: Cardiac Troponin I (cTnI)
Clonality: Monoclonal
Entrez Gene: 7137
Swiss Prot: P19429
Source: KLH conjugated synthetic peptide derived from human CTn1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
IHC-P(IHC-P(1:200-2000))
IHC-F(IHC-F(1:200-2000))
IF(IHC-P)(IF(IHC-P)(1:200-2000))
Predicted Molecular Weight: 23
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cardiac Troponin I (cTnI)) Monoclonal Antibody, Unconjugated (bsm-25443M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.