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Host: Mouse
Target Protein: human CD10
Clonality: Monoclonal
Isotype: Mouse IgG2b, k
Entrez Gene: 4311
Swiss Prot: P08473
Source: KLH conjugated synthetic peptide derived from human CD10
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS (pH7.4).
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
IF(ICC)(IF(ICC)(1:50-200))
FCM(FCM(1ug/Test))
Predicted Molecular Weight: 82
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD10) monoclonal Antibody, unconjugated (bsm-30012M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Ramos (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-human CD10 antibody (bsm-30012M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.