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Host: Mouse
Target Protein: human CD11a
Clonality: Monoclonal
Isotype: IgG2b/Kappa
Entrez Gene: 3683
Swiss Prot: P20701
Source: N/A
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
FCM(FCM(1ug/Test))
IF(ICC)(IF(ICC)(1:100-500))
Predicted Molecular Weight: 126
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Jurkat cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (human CD11a) monoclonal Antibody, Unconjugated (bsm-30013M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
scatter diagram showing human peripheral blood leukocytes stained with human CD11a. The cells were incubated with the antibody (bsm-30013M) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.