bsm-30016M
[Primary Antibody]
human CD15 (SSEA-1) Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: human CD15 (SSEA-1)
Clonality: Monoclonal
Isotype: IgG1 κ
Source: Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications: IHC-P(1:200-400)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.