support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Mouse
Target Protein: human CD18
Clonality: Monoclonal
Isotype: Mouse IgG1, k
Entrez Gene: 3689
Swiss Prot: P05107
Source: KLH conjugated synthetic peptide derived from human CD18
Purification: Purified by Protein G.
Storage Buffer: 0.01M PBS (pH7.4).
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
FCM(FCM(1:50-100))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
Predicted Molecular Weight: 82
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
The human blood granulocytes (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD18 antibody (bsm-30018M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with human CD18 Monoclonal Antibody, Unconjugated (bsm-30018M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with human CD18 Monoclonal Antibody, Unconjugated (bsm-30018M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.