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Host: Mouse
Target Protein: Human CD43
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 6693
Swiss Prot: P16150
Source: KLH conjugated synthetic peptide derived from human CD43
Purification: Purified by Protein A.
Storage Buffer: Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
ELISA(ELISA(1:5000-10000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IF(ICC)(IF(ICC)(1:100-500))
Predicted Molecular Weight: 40
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Human CD43) monoclonal Antibody, unconjugated (bsm-30037M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human CD43) Monoclonal Antibody, Unconjugated (bsm-30037M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human CD43) Monoclonal Antibody, Unconjugated (bsm-30037M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.