bsm-30157A [Primary Antibody]
mouse CD25 Monoclonal Antibody
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Host: Rat

Target Protein: mouse CD25

Clonality: Monoclonal

Isotype: Rat IgG1, λ

Entrez Gene: 3559

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100uL

Concentration: 1mg/ml

Applications: FCM(FCM(1ug/Test))

Predicted Molecular Weight: 28


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:ctll-2. Primary Antibody (green line): Rabbit Anti-mouse CD25 antibody (bsm-30157A) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-rat IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line):Normal Rat IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.