bsm-30162A [Primary Antibody]
mouse CD86 Monoclonal Antibody
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Host: Rat

Target Protein: mouse CD86

Clonality: Monoclonal

Isotype: Rat IgG2a, k

Entrez Gene: 56822

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100uL

Concentration: 1mg/ml

Applications: WB(WB(1:500-2000))
FCM(FCM(1ug/Test))

Predicted Molecular Weight: 31


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

scatter diagram showing mouse spleen stained with CD86.The cells were incubated with the antibody (bsm-30162A, 1 µg/Test) for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.Acquisition of >20,000 events was performed.


25 ug total protein per lane of various lysates (see on figure) probed with mouse CD86 monoclonal antibody, unconjugated (bsm-30162A) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.