bsm-33005M [Primary Antibody]
V5 tag Monoclonal Antibody
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Host: Mouse

Target Protein: V5 tag

Clonality: Monoclonal

Isotype: IgG

Source: KLH conjugated V5-tag peptide

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Cross Reactive Species: Species independent
(V5 tag)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

8ug Tags Positive Control Whole Cell Lysate, His (bs-41230P) per lane probed with V5 tag monoclonal antibody respectively, unconjugated (bsm-33005M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.