bsm-33018M [Primary Antibody]
Trx-Tag Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Trx-Tag

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7295

Swiss Prot: P10599

Source: Recombinant human Thioredoxin

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 12


Cross Reactive Species: Tags
Others
(fusion protein)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33018P); Lane 2: Control (-) E.coli lysates probed with Thioredoxin/Trx Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Recombined Trx/His-tag porcine GM-CSF protein; Lane 2: Enzyme digested Trx-tag probed with Trx-Tag Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.