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Host: Rabbit
Target Protein: beta-Actin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 60
Swiss Prot: P60709
Source: Synthetic MAP peptide derived from human beta-Actin
Purification: Purified by Protein G.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Conjugation: HRP
Excitation/ Emission: N/A
Size: 100uL
Concentration: 1ug/ul
Applications:
WB(WB=1:500-5000)
IHC-P(IHC-P=1:200-1000)
IHC-F(IHC-F=1:200-1000)
IF(IF=1:200-1000)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33032M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, HRP conjugated (bsm-33032M-HRP) at 1:5000 dilution and 4°C overnight incubation.