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Host: Rabbit
Target Protein: beta-Actin
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 60
Swiss Prot: P60709
Source: Synthetic MAP peptide derived from human beta-Actin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:20000-200000))
FCM(FCM(1:100-500))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Chicken
Rabbit
Hamster
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33032R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33032R) at 1:100000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33032R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33032R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33032R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33032R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-beta-Actin antibody (bsm-33032R,1:200); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.