bsm-33033M [Primary Antibody]
GAPDH Monoclonal Antibody
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Host: Mouse

Target Protein: GAPDH

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2597

Swiss Prot: P04406

Source: KLH conjugated synthetic peptide derived from human GAPDH

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat
Hamster

Predicted Cross Reactive Species: Dog
Sheep
Pig
Chicken
Rabbit
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cui et al. Chronic Heat Stress Induces Immune Response, Oxidative Stress Response, and Apoptosis of Finishing Pig Liver: A Proteomic Approach. (2016) Int.J.Mol.Sc. 17 Read more>>
  • Li Q et al. The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway.(2018) Lasers Med Sci. Sep 15Read more>>
  • Wang W et al. Proteomic Analysis on Exosomes Derived from Patients' Sera Infected with Echinococcus granulosus. Korean J Parasitol. 2019 Oct;57(5):489-497.Read more>>
  • Jiang S et al. Effect of Illicium verum or Eucommia ulmoides leaf extracts on the anti-stress ability, and mRNA and protein expression of Nrf2 and TNF-α in Duroc × Landrace × Yorkshire and Chinese native Licha-black nursery piglets. J Anim Physiol Anim Nutr (Berl). 2019 Oct 31. Read more>>
  • Zengyu Ma et al. Identification and functional characterization of ribosomal protein S23 as a new member of antimicrobial protein. Dev Comp Immunol. 2020 Sep;110:103730.Read more>>
  • Chunyu Zhang et al. WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF- signalling pathway. Mol Cell Biochem. 2020 Jul;470(1-2):215-227.Read more>>
  • Chao X et al. miR-429-3p/LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPAR PathwayFront Cell Dev Biol.2020 Dec 21;8:595637.Read more>>
  • Cao Y et al. Protective effect of carnosine on hydrogen peroxideCinduced oxidative stress in Human kidney tubular epithelial cellsBiochem Biophys Res Commun.2021 Jan 1;534:576-582.Read more>>
  • Nier Cha. et al. MicroRNA?124 suppresses the invasion and proliferation of breast cancer cells by targeting TFAP4. Oncol Lett. 2021 Apr;21(4):1-1Read more>>
  • Ping Gong. et al. p47 phox deficiency improves cognitive impairment and attenuates tau hyperphosphorylation in mouse models of AD. Alzheimers Res Ther. 2020 Dec;12(1):1-18Read more>>
  • Yamashita Koichiro. et al. Mitotic phosphorylation of Pex14p regulates peroxisomal import machinery. J Cell Biol. 2020 Oct;219(10):e202001003.Read more>>
  • Yaodong Zhou. et al. Association of MicroRNA-21 with p53 at Mutant Sites R175H and R248Q, Clinicopathological Features, and Prognosis of NSCLC. Mol Ther-Oncolytics. 2020 Dec;19:208Read more>>
  • Xiaohui Pan. et al. miR-1297 Suppresses Osteosarcoma Proliferation and Aerobic Glycolysis by Regulating PFKFB2. Oncotargets Ther. 2020; 13: 11265C11275Read more>>
  • Wenshuang Liao. et al. Illicium verum and Eucommia ulmoides leaf extracts promote nutrient availability and antioxidant capacity in piglets by upregulating duodenal and jejunal Nrf2/TNF\. 2021 Mar 26Read more>>
  • Lijin Guo. et al. Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis. Front Cell Dev Biol. 2021; 9: 653798Read more>>
  • Xueli Bai. et al. Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Oct;99:107996Read more>>
  • Yongfeng Pang. et al. Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimers Disease in Mice by Modulating the miR-567/NEUROD2/PSD95 Axis. Neuropsych Dis Treat. 2021; 17: 2151C2161Read more>>
  • Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153Read more>>
  • Yansiwei Cheng. et al. Simulated Microgravity Inhibits Rodent Dermal Fibroblastic Differentiation of Mesenchymal Stem Cells by Suppressing ERK/-Catenin Signaling Pathway. Int J Mol Sci. 2021 Jan;22(19):10702Read more>>
  • Shunshun Han. et al. Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken. Poultry Sci. 2021 Oct;:101524Read more>>
  • Yue SU. et al. Exosomes derived from placental trophoblast cells regulate endometrial epithelial receptivity in dairy cows during pregnancy. 2021 Oct 22Read more>>
  • Francesca Rossi. et al. Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity. Mol Cell. 2022 Jan;82:75Read more>>
  • Peng Gu. et al. Suppression of CDCA3 inhibits prostate cancer progression via NF?B/cyclin D1 signaling inactivation and p21 accumulation. Oncol Rep. 2022 Feb;47(2):1-1Read more>>
  • Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUC-MSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15Read more>>
  • Zhe Song. et al. Isoandrographolide inhibits NLRP3 inflammasome activation and attenuates silicosis in mice. Int Immunopharmacol. 2022 Apr;105:108539Read more>>
  • Quanwei Li. et al. Toxicological mechanism of large amount of copper supplementation: Effects on endoplasmic reticulum stress and mitochondria-mediated apoptosis by Nrf2/HO-1 pathway-induced oxidative stress in the porcine myocardium. J Inorg Biochem. 2022 May;230:11175Read more>>
  • Guo-Dong Wu. et al. Cordyceps Improves Obesity and its Related Inflammation via Modulation of Enterococcus cecorum Abundance and Bile Acid Metabolism. 2022 Mar 1Read more>>
  • Hongxiao Yang. et al. Hydrogen Attenuates Thyroid Hormone-Induced Cardiac Hypertrophy in Rats by regulating angiotensin II type 1 receptor and NADPH oxidase 2 mediated oxidative stress. Eur J Pharmacol. 2022 May;922:174917Read more>>
  • Jiawei Mo. et al. Construction and Analysis of Disuse Atrophy Model of the Gastrocnemius Muscle in Chicken. INT J MOL SCI. 2022 Jan;23(13):6892Read more>>
  • Rao XL. et al. Neuroprotective effects of visnagin on cerebral ischemia-reperfusion injury rats and the underlying mechanisms.. EUR REV MED PHARMACO. 2022 Jun;26(12):4371-4379Read more>>
  • Junxuan Wu. et al. Cathepsin B/HSP70 complex induced by Ilexsaponin I suppresses NLRP3 inflammasome activation in myocardial ischemia/reperfusion injury. PHYTOMEDICINE. 2022 Jul;:154358Read more>>
  • Juan Feng. et al. Melatonin prevents cyclophosphamide-induced primordial follicle loss by inhibiting ovarian granulosa cell apoptosis and maintaining AMH expression. FRONT ENDOCRINOL. 2022; 13: 895095Read more>>
  • You Li. et al. LncRNA PVT1 is a novel mediator promoting the angiogenesis response associated with collateral artery formation. INT J BIOCHEM CELL B. 2022 Aug;:106294Read more>>
  • Haijun Sun. et al. WD Repeat Domain 43 promotes malignant progression of non-small cell lung cancer by regulating CDK2. INT J BIOCHEM CELL B. 2022 Aug;:106293Read more>>
  • Lijin Guo. et al. Chicken Protein S Gene Regulates Adipogenesis and Affects Abdominal Fat Deposition. ANIMALS. 2022 Jan;12(16):2046Read more>>
  • Rui Guo. et al. Ulinastatin attenuates spinal cord injury by targeting AMPK/NLRP3 signaling pathway. J CHEM NEUROANAT. 2022 Nov;125:102145Read more>>
  • Ji-Soo Oh. et al. Synthetic TGF-β Signaling Agonist-Treated Dendritic Cells Induce Tolerogenicity and Antirheumatic Effects. CURR ISSUES MOL BIOL. 2022 Sep;44(9):3809-3821Read more>>
  • Ling Guo. et al. Calcicoptosis induced by purple sweet potato anthocyanins through the nonosmotic regulation of the NFAT5/S100A4-S100A9 pathway in acute lymphoblastic leukemia. CHEM BIODIVERS. 2022 AugRead more>>
  • Shuang Chen. et al. Melatonin activates the Mst1-Nrf2 signaling to alleviate cardiac hypertrophy in pulmonary arterial hypertension. EUR J PHARMACOL. 2022 Sep;:175262Read more>>
  • Kunlin Wu. et al. Histone deacetylase inhibitor panobinostat in combination with rapamycin confers enhanced efficacy against triple-negative breast cancer. EXP CELL RES. 2022 Sep;:113362Read more>>
  • Zhuoying Hu. et al. MitomiR-504 alleviates the copper-induced mitochondria-mediated apoptosis by suppressing Bak1 expression in porcine jejunal epithelial cells. SCI TOTAL ENVIRON. 2023 Feb;858:160157Read more>>
  • He Bai. et al. Inhibition of the BNIP3/NIX-dependent mitophagy aggravates copper-induced mitochondrial dysfunction in duck renal tubular epithelial cells. ENVIRON TOXICOL. 2022 NovRead more>>
  • Ying Yang. et al. Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3 to regulate macrophage/microglial polarization. PHYTOMEDICINE. 2022 Dec;:154602Read more>>
  • Rushuang Xu. et al. Plectin Downregulation Inhibits Migration and Suppresses Epithelial Mesenchymal Transformation of Hepatocellular Carcinoma Cells via ERK1/2 Signaling. INT J MOL SCI. 2023 Jan;24(1):73Read more>>
  • Li Kan. et al. m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte. Journal of Animal Science and Biotechnology. 2022 Dec;13(1):1-15Read more>>
  • aoxin Qiao. et al. Curcumin attenuates AFB1-induced duck liver injury by inhibiting oxidative stress and lysosomal damage. FOOD CHEM TOXICOL. 2022 Dec;:113593Read more>>
  • Liangliang Zhang. et al. Inhibition of EZH2 Causes Retrotransposon Derepression and Immune Activation in Porcine Lung Alveolar Macrophages. INT J MOL SCI. 2023 Jan;24(3):2394Read more>>
  • Meng Li. et al. CircRNA Profiling of Skeletal Muscle in Two Pig Breeds Reveals CircIGF1R Regulates Myoblast Differentiation via miR-16. INT J MOL SCI. 2023 Jan;24(4):3779Read more>>
  • Sun Qingyun. et al. Glucose-induced enhanced antioxidant activity inhibits apoptosis in goose fatty liver. J ANIM SCI. 2023 FebRead more>>
  • Duan Xiaojiang. et al. First-in-human study of the radioligand 68Ga-N188 targeting nectin-4 for PET/CT imaging of advanced urothelial carcinoma. CLIN CANCER RES. 2023 ApRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, DAB staining.


25 ug total protein per lane of various lysates (see on figure) probed with GAPDH monoclonal antibody, unconjugated (bsm-33033M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.