VALIDATION IMAGES
Lane 1: U87mg lysates; Lane 2: SW480 lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-33034M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:400 overnight at 4°C, DAB staining.
Lane 1: HL60 lysates;Lane 2: U937 lysates;Lane 3: A431 lysates; Lane 4: SH-SY5Y lysates probed with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3:HL60 cell lysates; Lane 4: SH-SY5Y cell lysates ; Lane 5:Mouse Cerebellum cell lysates;Lane 6:Rat Cerebellum cell lysates;7: Mouse Cerebrum cell lysates;Lane 8: Rat Cerebrum cell lysates probed with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:2000 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:2000 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, DAB staining.
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary:
Beta tubulin (bsm-33034M) at 1/1000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary:
Beta tubulin (bsm-33034M) at 1/1000~20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Sample:
Hela (Human) Lysate at 40 ug
Primary:
Beta tubulin (bsm-33034M) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution