bsm-33034R-HRP [Conjugated Primary Antibody]
Beta tubulin Monoclonal Antibody, HRP Conjugated
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Host: Rabbit

Target Protein: Beta tubulin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 203068

Swiss Prot: P07437

Source: KLH conjugated synthetic peptide derived from human Beta tubulin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:50000-200000))
FCM(FCM(1μg/Test))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
IF(ICC)(IF(ICC)(1:50-200))

Predicted Molecular Weight: 50


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Beta tubulin monoclonal antibody, HRP conjugated (bsm-33034R-HRP) at 1:50000 dilution and 4°C overnight incubation.


25 ug total protein per lane of various lysates (see on figure) probed with Beta tubulin monoclonal antibody, HRP conjugated (bsm-33034R-HRP) at 1:10000 dilution and 4°C overnight incubation.