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Host: Rabbit
Target Protein: PCNA
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5111
Swiss Prot: P12004
Source: KLH conjugated synthetic peptide derived from human PCNA
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Conjugation: HRP
Excitation/ Emission: N/A
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:5000-20000))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
Predicted Molecular Weight: 29
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035R-HRP) at 1:1000 dilution and 4°C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.