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Host: Rabbit
Target Protein: PCNA
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5111
Swiss Prot: P12004
Source: KLH conjugated synthetic peptide derived from human PCNA
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Conjugation: HRP
Excitation/ Emission: N/A
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(WB(1:5000-20000))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
Predicted Molecular Weight: 29
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035R-HRP) at 1:1000 dilution and 4°C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PCNA Monoclonal Antibody (bsm-33035R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.