bsm-33036M [Primary Antibody]
beta-Actin Monoclonal Antibody
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support@biossusa.com
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DATASHEET

Host: Mouse

Target Protein: beta-Actin

Specificity: The immunogen of this antibody has sequence similarity to actin cytoplasmic 2 and beta-actin-like protein 2, and may bind to these targets.

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 60

Swiss Prot: P60709

Source: KLH conjugated synthetic peptide derived from human beta-Actin

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Size: 100µL

Concentration: Lot Dependent

Applications: WB
IHC-P(1:100-500)
IHC-F(1:200-400)
IF(1:300-5000)

Predicted Molecular Weight: 42kDa


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xuan et al. Short?term vagus nerve stimulation reduces myocardial apoptosis by downregulating microRNA?205 in rats with chronic heart failure. (2017) Mol.Med.Rep. 16:5847-5854Read more>>
  • Zeng et al. Resveratrol improves prostate fibrosis during progression of urinary dysfunction in chronic prostatitis by mast cell suppression. (2018) Mol.Med.Rep. 17:918-924Read more>>
  • Liu et al. Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression. (2014) PLoS.On. 9:e98560Read more>>
  • Zhang P et al. MicroRNA-143a-3p modulates preadipocyte proliferation and differentiation by targeting MAPK7. Biomedicine & Pharmacotherapy, 108, 531–539. Read more>>
  • Li, J., et al. Cellular microRNA bta-miR-222 suppresses caprine parainfluenza virus type 3 replication via downregulation of interferon regulatory factor 2.(2018) Veterinary Microbiology Read more>>
  • Wang X et al. Inhibition of microRNA-182-5p contributes to attenuation of lupus nephritis via Foxo1 signaling. (2018) Exp Cell Res. pii: S0014-4827(18)31038-3.Read more>>
  • Liu Z et al. The neuroprotective effect of lithium chloride on cognitive impairment through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats.(2018) Eur J Pharmacol. 2018 Dec 5;840:50-59.Read more>>
  • Guo F et al. miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells.(2018) Clin Exp Pharmacol Physiol. Oct 24.Read more>>
  • Si J et al. Linagliptin protects rat carotid artery from balloon injury and activates the NRF2 antioxidant pathway.(2018) Exp Anim. Oct 23.Read more>>
  • Pang X et al. The Role of IL-6RA in UHMWPE Promotes Proliferation in Fibro-Like Synovial Cells. (2018) Biomed Res Int. 2018 Oct 22;2018:3928915. Read more>>
  • Tang et al. Co-Upregulation of 14-3-3ζ and P-Akt is Associated with Oncogenesis and Recurrence of Hepatocellular Carcinoma. (2018) Cell.Physiol.Biochem. 45:1097-1107Read more>>
  • Wang R et al. Iron-induced oxidative stress contributes to α-synuclein phosphorylation and up-regulation via polo-like kinase 2 and casein kinase 2. Neurochem Int. 2019 May;125:127-135.Read more>>
  • Xue T et al. A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro. BMC Vet Res. 2019 Feb 1;15(1):47.Read more>>
  • Liu LB et al. SXBX pill suppresses homocysteine-induced vascular smooth muscle cells dedifferentiation by inhibiting NLRP3 inflammasomes activation via ERK/p38 MAPK pathways.Am J Transl Res. 2019 Feb 15;11(2):806-818. Read more>>
  • "Chen X et al. Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.BioMed Research International.2019 May;1-8. "Read more>>
  • Wang L et al. LncRNA HCP5 promotes triple negative breast cancer progression as a ceRNA to regulate BIRC3 by sponging miR‐219a‐5p. Cancer Med. 2019 Jun 18.Read more>>
  • Yang YD et al. Targeting mitochondria‐associated membranes as a potential therapy against endothelial injury induced by hypoxia. J Cell Biochem. 2019 Jun 26.Read more>>
  • Yue M et al. Effects of body temperature on the expression and localization of meiosis-related proteins STRA8 and SCP3 in boar testes. Acta Histochem. 2019 Aug;121(6):718-723.Read more>>
  • Deng W et al. Insulin ameliorates pulmonary edema through the upregulation of epithelial sodium channel via the PI3K/SGK1 pathway in mice with lipopolysaccharide‑induced lung injury. Mol Med Rep. 2019 Mar;19(3):1665-1677. Read more>>
  • Wei Q et al. Expression of Dual-Specificity Phosphatase 9 in Placenta and Its Relationship with Gestational Diabetes Mellitus. Journal of Diabetes Research, 2019, 1–7. Read more>>
  • Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. Read more>>
  • Xu G et al. Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish. Sci Rep. 2019 Dec 11;9(1):18910. Read more>>
  • Wang HG et al. Synergistic Lethality Between PARP-trapping and Alantolactone-Induced Oxidative DNA Damage in Homologous Recombination-Proficient Cancer Cells. Oncogene . 2020 Apr;39(14):2905-2920.Read more>>
  • Feng Y et al. E2F4 functions as a tumour suppressor in acute myeloid leukaemia via inhibition of the MAPK signalling pathway by binding to EZH2. J Cell Mol Med. 2020 Jan 14.Read more>>
  • Liu Y et al. Expression and localization of meiosis-associated protein in gonads of female rats at different stages. Acta Histochem. 2020 Jan 18:151509.Read more>>
  • Feng Y et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol. 2020 Mar 26;8:142. Read more>>
  • Wang HG et al. Synergistic Lethality Between PARP-trapping and Alantolactone-Induced Oxidative DNA Damage in Homologous Recombination-Proficient Cancer Cells. Oncogene . 2020 Apr;39(14):2905-2920.Read more>>
  • Houpan Song et al. Traditional Chinese Medicine Li-Zhong-Tang accelerates the healing of indomethacin-induced gastric ulcers in rats by affecting TLR-2/MyD88 signaling pathway. J Ethnopharmacol. 2020 Sep 15;259:112979.Read more>>
  • Honghe Xiao et al. Shenzao jiannao oral liquid, an herbal formula, ameliorates cognitive impairments by rescuing neuronal death and triggering endogenous neurogenesis in AD-like mice induced by a combination of A42 and scopolamine. J Ethnopharmacol. 2020 Sep 15;259:112957.Read more>>
  • Zhang F et al. Affibody\Conjugated RALA Polymers Delivering Oligomeric 5\Fluorodeoxyuridine for Targeted Therapy of HER2 Overexpressing Gastric Cancer. Macromol Biosci. 2020 Jul;20(7):e2000083.Read more>>
  • Li K et al. Recombinant Human Elafin Ameliorates Chronic Hyperoxia-Induced Lung Injury by Inhibiting Nuclear Factor-Kappa B Signaling in Neonatal Mice. J Interferon Cytokine Res. 2020 Jun;40(6):320-330.Read more>>
  • Hongxi Wuet al. Loss of a Negative Feedback Loop between IRF8 and AR Promotes Prostate Cancer Growth and Enzalutamide Resistance. Cancer Res. 2020 Jul 1;80(13):2927-2939.Read more>>
  • Feng Y et al. Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML)Cell Death Dis.2020 Dec 2;11(12):1025.Read more>>
  • Xiong Y et al. Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirdsPLoS Genet.2020 Dec 28;16(12):e1009270.Read more>>
  • Jingsong Xiao. et al. PGC-1 Mediated-EXOG, a Specific Repair Enzyme for Mitochondrial DNA, Plays an Essential Role in the Rotenone-Induced Neurotoxicity of PC12 Cells. 2021 Jan 3Read more>>
  • Xiong Ying. et al. SLC2A12 of SLC2 Gene Family in Bird Provides Functional Compensation for the Loss of SLC2A4 Gene in Other Vertebrates. Mol Biol Evol. 2020 NovRead more>>
  • Xing Xue. et al. Effects of local dexmedetomidine administration on the neurotoxicity of ropivacaine for sciatic nerve block in rats. Mol Med Rep. 2020 Nov;22(5):4360-4366Read more>>
  • Jisheng Wang. et al. Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats. Med Sci Monitor. 2020; 26: e925579-1Ce925579-11Read more>>
  • Meng-Qi Wu. et al. High-mobility group box1 as an amplifier of immune response and target for treatment in Aspergillus fumigatus keratitis. Int J Ophthalmol-Chi. 2020; 13(5): 708C717Read more>>
  • Shuangshuang Kang. et al. Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives. 2021 Mar 22Read more>>
  • Yang Liu. et al. Insulin Resistance and Pellino-1 Mediated Decrease in the Activities of Vasodilator Signaling Contributes to Sunitinib-Induced Hypertension. Front Pharmacol. 2021; 12: 617165Read more>>
  • Xiao-Lie He. et al. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res. 2021 Mar;16(3):537Read more>>
  • Fang Wang. et al. Stratifin promotes renal dysfunction in ischemic and nephrotoxic AKI mouse models via enhancing RIPK3-mediated necroptosis. Acta Pharmacol Sin. 2021 Apr;:1-12Read more>>
  • Lun Yao. et al. Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells. Viruses-Basel. 2021 Apr;13(4):691Read more>>
  • Zijian Geng. et al. LPS-induced SOCS3 antagonizes the JAK2-STAT5 pathway and inhibits -casein synthesis in bovine mammary epithelial cells. Life Sci. 2021 Aug;278:119547Read more>>
  • Haifa Alkhalifa. et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett. 2021 Jun;21(6):1-11Read more>>
  • Zhuohong Li. et al. Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-B activity. 2021 May 06Read more>>
  • Chao Ye. et al. ATP-dependent activation of NLRP3 inflammasome in primary murine macrophages infected by pseudorabies virus. Vet Microbiol. 2021 Aug;259:10913Read more>>
  • Shuyu Hu. et al. The effect of Wnt/-catenin pathway on the scleral remolding in the mouse during form deprivation. 2021 May 13Read more>>
  • ingxiang Wang. et al. Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4. J Lipid Res. 2021 Jun;:100091Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.


25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33036M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated(bsm-33036M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated(bsm-33036M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of bsm-33036M) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of bsm-33036M) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of bsm-33036M) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of bsm-33036M) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.