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Host: Mouse
Target Protein: alpha Tubulin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7277
Swiss Prot: P68366
Source: Recombinant human alpha Tubulin Protein
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:5000-100000))
FCM(FCM(1:50-100))
IHC-P(IHC-P(1:500-1000))
IHC-F(IHC-F(1:500-1000))
IF(IHC-P)(IF(IHC-P)(1:500-1000))
Predicted Molecular Weight: 50
Cross Reactive Species:
Human
Mouse
Rat
Zebrafish
Predicted Cross Reactive Species:
Dog
Pig
Chicken
Rabbit
GuineaPig
For research use only. Not intended for diagnostic or therapeutic use.
- Yaying Wang. et al. Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak.. FRONT VET SCI. 2022 Aug;9:960250-96025Read more>>
- Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 ApRead more>>
- Yingjun Zhou. et al. Auricularia auricula-judae (Bull.) polysaccharides improve obesity in mice by regulating gut microbiota and TLR4/JNK signaling pathway. INT J BIOL MACROMOL. 2023 Oct;250:126172Read more>>
- Junling Gao. et al. Mechanisms underlying the synergistic effects of chuanxiong combined with Chishao on treating acute lung injury based on network pharmacology and molecular docking combined with preclinical evaluation. J ETHNOPHARMACOL. 2024 May;325:117862Read more>>
- Sibo Wang. et al. Design, synthesis and biological evaluation of novel tubulin-targeting agents with a dual-mechanism for polymerization inhibition and protein degradation. EUR J MED CHEM. 2024 Jun;272:116458Read more>>
25 ug total protein per lane of various lysates (see on figure) probed with alpha Tubulin monoclonal antibody, unconjugated (bsm-33039M) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Tubulin Monoclonal Antibody, Unconjugated (bsm-33039M) at 1:1000 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-alpha Tubulin antibody (bsm-33039M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.