bsm-33041M [Primary Antibody]
beta 1 Tubulin (5F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: beta 1 Tubulin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 81027

Swiss Prot: Q9H4B7

Source: KLH conjugated synthetic peptide derived from human beta I Tubulin

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ruihua Xu. et al. Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells. ANIMALS. 2022 Jan;12(22):3174Read more>>
  • Rui Zhang. et al. CIRBP Increases the Synthesis and Secretion of Steroid Hormones by Activating Autophagy in Yak Granule Cells. J STEROID BIOCHEM. 2023 Dec;:106449Read more>>
VALIDATION IMAGES

Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1：Human 293T cell lysates; Lane 2: Mouse Cerebellum lysates; Lane 3:Human Jurkat cell lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Fetal brain lysates; Lane 5: Mouse Heart lysates; Lane 6: Human SH-SY5Y cell lysates; Lane 7: Rat Spleen lysates; Lane 8: Mouse Spleen lysates; Lane 9: Rat Ovary lysates probed with beta 1 Tubulin (5F7) Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.