bsm-33042M-HRP [Conjugated Primary Antibody]
Histone H3 Monoclonal Antibody, HRP Conjugated
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Host: Mouse

Target Protein: Histone H3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8290

Swiss Prot: Q16695

Source: Recombinant human HIST3H3 Protein

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tian, Yue, et al. "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." International Journal of Molecular Medicine.Read more>>
  • Zhao, Dongmei, et al. "Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro." Experimental and Therapeutic Medicine.Read more>>
  • Zhao et al. Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damagein vivoandin vitro. (2017) Exp.Ther.Med. 14:5515-5522Read more>>
  • Dong R et al. Temporospatial effects of acyl‐ghrelin on activation of astrocytes after ischemic brain injury. J Neuroendocrinol. 2019 Jul 5:e12767. Read more>>
  • Zheng GH et al. Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxid Med Cell Longev. 2019 Jan 22;2019:5189819. Read more>>
  • Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE ?/? mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.Read more>>
  • Lei Chen. et al. Perillaldehyde: A promising antifungal agent to treat oropharyngeal candidiasis. Biochem Pharmacol. 2020 Oct;180:114201Read more>>
  • Qing Shu. et al. Mechanisms by which electroacupuncture?mediated histone acetylation mitigates bone loss in rats with ovariectomy?induced osteoporosis. Mol Med Rep. 2020 Oct;22(4):3453-3463Read more>>
  • Chu Xuan. et al. DNMT3A R882H mutation drives daunorubicin resistance in acute myeloid leukemia via regulating NRF2/NQO1 pathway. CELL COMMUN SIGNAL. 2022 Dec;20(1):1-14Read more>>
  • Hongying Dong. et al. Pueraria lobata antioxidant extract ameliorates non-alcoholic fatty liver by altering hepatic fat accumulation and oxidative stress. J ETHNOPHARMACOL. 2024 Oct;333:118468Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Histone H3/HIST3H3/HRP monoclonal antibody, conjugated (bsm-33042M-HRP) at 1:50000 dilution and 4°C overnight incubation.