bsm-33042R-Biotin [Conjugated Primary Antibody]
Histone H3 Monoclonal Antibody, Biotin Conjugated
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Host: Rabbit

Target Protein: Histone H3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8968

Swiss Prot: Q71DI3

Source: KLH conjugated synthetic peptide derived from human Histone H3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:5000-50000))
IHC-P(IHC-P(1:500-2000))
IHC-F(IHC-F(1:500-2000))

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Bee
Hamster

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.