bsm-33042R-PE [Conjugated Primary Antibody]
Histone H3 Monoclonal Antibody, PE Conjugated
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Host: Rabbit

Target Protein: Histone H3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8968

Swiss Prot: Q71DI3

Source: KLH conjugated synthetic peptide derived from human Histone H3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:5000-50000))
FCM(FCM(1μg/Test))
IHC-P(IHC-P(1:500-2000))
IHC-F(IHC-F(1:500-2000))
IF(IHC-P)(IF(IHC-P)(1:500-2000))
IF(ICC)(IF(ICC)(1:100-500))
ChIP(ChIP(6μg/IP))

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Bee
Hamster

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): PE conjugated Rabbit Anti-Histone H3 antibody (bsm-33042R-PE): 1 μg/10^6 cells;Isotype Control (orange): Rabbit IgG/PE (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.


4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Histone H3) monoclonal Antibody, conjugated (bsm-33042R-PE) 1:100, 90 min at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.