bsm-33047M
[Primary Antibody]
Bcl2 Monoclonal Antibody
DATASHEET
Host: Mouse
Target Protein: Bcl2
Immunogen Range: 51-150/239
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 596
Swiss Prot: P10415
Source: KLH conjugated synthetic peptide derived from human Bcl2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Liu Z et al. The neuroprotective effect of lithium chloride on cognitive impairment through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats.(2018) Eur J Pharmacol. 2018 Dec 5;840:50-64.Read more>>
- Zhao TY et al. Recovery of Chemotherapy-induced Anemia by Angelica Polysaccharides in C57BL/6N Mice and Elucidation of Underlying Mechanism. Pakistan J. Zool. 2019 51, 5:1927-1935.Read more>>
- Zhang F et al. Affibody\Conjugated RALA Polymers Delivering Oligomeric 5\Fluorodeoxyuridine for Targeted Therapy of HER2 Overexpressing Gastric Cancer. Macromol Biosci. 2020 Jul;20(7):e2000083.Read more>>
- Li R et al. Destruction of Neutrophil Extracellular Traps Promotes the Apoptosis and Inhibits the Invasion of Gastric Cancer Cells by Regulating the Expression of Bcl-2, Bax and NF-B. Onco Targets Ther. 2020 Jun 9;13:5271-5281.Read more>>
- Jingwei Song. et al. Swine MicroRNAs ssc-miR-221-3p and ssc-miR-222 Restrict the Cross-Species Infection of Avian Influenza Virus. J Virol. 2020 Nov;94(23):e01700-2Read more>>
- Chunyue Wang. et al. Isoforsythiaside Attenuates Alzheimers Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway. Int J Mol Sci. 2020 Jan;21(16):5687Read more>>
- Chao Zhang. et al. Affibody Modified G-quadruplex DNA Micelles Incorporating Polymeric 5-Fluorodeoxyuridine for Targeted Delivery of Curcumin to Enhance Synergetic Therapy of HER2 Positive Gastric Cancer. Nanomaterials-Basel. 2022 Jan;12(4):696Read more>>
- Guodong Zhao. et al. Isolation and Characterization of Natural Nanoparticles in Naoluo Xintong Decoction and Their Brain Protection Research. Molecules. 2022 Jan;27(5):1511Read more>>
- Jingsong Liu. et al. The anti-apoptotic and anti-autophagic effects of EPO through PI3K/Akt/mTOR signaling pathway in MAC-T cells. RES VET SCI. 2022 Dec;149:1Read more>>
- Guangmin Li. et al. Chiral FA Conjugated CdTe/CdS Quantum Dots for Selective Cancer Ablation. ACS NANO. 2022;16(8):1299113001Read more>>
- Pin-Shi Ni. et al. Indirect regulation of HIPPO pathway by miRNA mediates high-intensity intermittent exercise to ameliorate aging skeletal muscle function. SCAND J MED SCI SPOR. 2023 FebRead more>>
- Anqi Yang. et al. FZD7, Regulated by Non-CpG Methylation, Plays an Important Role in Immature Porcine Sertoli Cell Proliferation. INT J MOL SCI. 2023 Jan;24(7):6179Read more>>
VALIDATION IMAGES
Lane 1: LOVO lysates; Lane 2: MCF7 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-33047M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: HL60 cell lysates probed with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl2) Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl2) Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl2) Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Human LOVO cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bsm-33047M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.