bsm-33048M [Primary Antibody]
HSP70 (5G10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: HSP70

Specificity: This antibody will recognize and HSPA1A and HSPA1B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3303, 3304

Swiss Prot: P0DMV9, P0DMV8

Source: KLH conjugated synthetic peptide derived from human HSP70

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cui et al. Chronic Heat Stress Induces Immune Response, Oxidative Stress Response, and Apoptosis of Finishing Pig Liver: A Proteomic Approach. (2016) Int.J.Mol.Sc. 17 Read more>>
  • Won Seob Kim. et al. Exploring the impact of temporal heat stress on skeletal muscle hypertrophy in bovine myocytes. J THERM BIOL. 2023 Aug;:103684Read more>>
VALIDATION IMAGES

HeLa cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


HepG2 cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with HSP70 (5G10) Monoclonal Antibody, Unconjugated (bsm-33048M) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.