bsm-33057M-Biotin [Conjugated Primary Antibody]
Cytokeratin 19 Monoclonal Antibody, Biotin Conjugated
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Cytokeratin 19

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3880

Swiss Prot: P08727

Source: KLH conjugated synthetic peptide derived from human Cytokeratin 19

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 44 kDa


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qi Y et al. Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice. Burns. 2019 May 6. Read more>>
  • Zequan Ding. et al. CTHRC1 serves as an indicator in biliary atresia for evaluating the stage of liver fibrosis and predicting prognosis. DIGEST LIVER DIS. 2024 JulRead more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Cytokeratin 19 monoclonal antibody, Biotin conjugated (bsm-33057M-Bio) at 1:1000 dilution and 4°C overnight incubation. Followed by HRP conjugated Streptavidin (bs-0437P-HRP) incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.