bsm-33058M [Primary Antibody]
p53 Monoclonal Antibody
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Host: Mouse

Target Protein: p53

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: KLH conjugated synthetic peptide derived from human p53

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shen S et al. Transcription factor early growth response-1 plays an oncogenic role in salivary gland pleomorphic adenoma. Biotechnol Lett. 2019 Nov 30.Read more>>
  • Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2?cells. J Ethnopharmacol. 2020 Dec 5;263:113072.Read more>>
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Cui Yuanbo. et al. RNA m6A demethylase FTO-mediated epigenetic up-regulation of LINC00022 promotes tumorigenesis in esophageal squamous cell carcinoma. J Exp Clin Canc Res. 2021 Dec;40(1):1-2Read more>>
  • Lin B. et al. Antioxidant Effects of <i>Sophora davidi</i> (Franch.) Skeels on d-Galactose-Induced Aging Model in Mice <i>via</i> Activating the SIRT1/p53 Pathway.. Front Pharmacol. 2021 Dec;12:754554-754554Read more>>
  • Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863Read more>>
  • Wang, Rui. et al. Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells. 2021 Nov 15Read more>>
  • Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-_B pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166Read more>>
  • Yuge Liao. et al. Inorganic mercury induces liver oxidative stress injury in quails by inhibiting Akt/Nrf2 signal pathway. INORG CHEM COMMUN. 2022 Aug;142:109603Read more>>
  • Xu Kejia. et al. Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles. REGEN BIOMATER. 2022 JulRead more>>
  • Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435Read more>>
  • Tianyu Yang. et al. Material properties of degradable alloy FeC30MnC0.6N and its effect on ferroptosis in synoviocytes. J BIOMED MATER RES B. 2022 SepRead more>>
  • Li, Guang. et al. Synergetic delivery of artesunate and isosorbide 5-mononitrate with reduction-sensitive polymer nanoparticles for ovarian cancer chemotherapy. J NANOBIOTECHNOL. 2022 Dec;20(1):1-13Read more>>
  • Hongyan Yu. et al. TBBPA rather than its main derivatives enhanced growth of endometrial cancer via p53 ubiquitination. J ENVIRON SCI-CHINA. 2022 DecRead more>>
  • Xu Chen. et al. Chiral Ruthenium Nanozymes with Self-Cascade Reaction Driven the NO Generation Induced Macrophage M1 Polarization Realizing the Lung Cancer Cocktail Therapy. SMALL. 2023 Apr;:2207823Read more>>
  • Ye Shen. et al. Influence of miR-142-3p on Pulmonary Fibrosis Through Regulation of p53/NF-B. J PHARMACOL PHARMACO. ;()Read more>>
  • Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
  • Reem M. Hazem. et al. Antitumor effect of trimetazidine in a model of solid Ehrlich carcinoma is mediated by inhibition of glycolytic pathway and AKT signaling. CHEM-BIOL INTERACT. 2023 Sep;383:110672Read more>>
  • Derya Erisik. et al. Differences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implications. ACS OMEGA. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Rui Liu. et al. Novel circular RNA hsa_circ_0036683 suppresses proliferation and migration by mediating the miR-4664-3p/CDK2AP2 axis in non-small cell lung cancer. THORAC CANCER. 2024 AugRead more>>
  • Xi-Kun Yuan. et al. Effects of Life-Long Exercise on Age-Related Inflammation, Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP 1/Klotho Pathway in Rat Kidneys. PHYSIOL RES. 2024 Aug 31;73(4):577-591Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with P53 monoclonal antibody, unconjugated (bsm-33058M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with P53 Monoclonal Antibody, Unconjugated (bsm-33058M) at 1:600 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with P53 Monoclonal Antibody, Unconjugated (bsm-33058M) at 1:600 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with P53 Monoclonal Antibody, Unconjugated (bsm-33058M) at 1:600 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.