Host: Mouse
Target Protein: GFAP
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 14580
Swiss Prot: P03995
Source: Recombinant mouse GFAP full length
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Predicted Molecular Weight: 50
Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Yukari Nagakura. et al. Expression of nischarin, an imidazoline 1 receptor candidate protein, in the ventrolateral medulla of newborn rats. Neurosci Lett. 2021 Jul;:136113Read more>>
- Qiuyun Tian. et al. Hypoxia-sensing VGLL4 promotes LDHA-driven lactate production to ameliorate neuronal dysfunction in a cellular model relevant to Alzheimer's disease. FASEB J. 2023 Nov;37(12):e2329Read more>>
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:400 overnight at 4°C, DAB staining.
Mouse Cerebellum lysates probed with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, DAB staining.