bsm-33085M [Primary Antibody]
C-erbB-2/HER2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: C-erbB-2/HER2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2064

Swiss Prot: P04626

Source: KLH conjugated synthetic peptide derived from human C-erbB-2/HER2

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 138 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liyun Guan. et al. Expression and clinical significance of HER2/neu, aromatase P450 and adhesion molecule CD24 in endometrial cancer. EUR J HISTOCHEM. 2023 Jul 26; 67(3): 3655Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C-erbB-2/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C-erbB-2/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4°C, DAB staining.


A549 cell lysates probed with C-erbB-2/HER2 Polyclonal Antibody, Unconjugated (bsm-33085M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with C-erbB-2/HER2 Polyclonal Antibody, Unconjugated (bsm-33085M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.