bsm-33101M [Primary Antibody]
KRT18 (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: KRT18

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3875

Swiss Prot: P05783

Source: Recombinant human KRT18

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qiushuang Sun. et al. Regulation on Citrate Influx and Metabolism through Inhibiting SLC13A5 and ACLY: A Novel Mechanism Mediating the Therapeutic Effects of Curcumin on NAFLD. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Xiuyun Xu. et al. Sox9+ cells are required for salivary gland regeneration after radiation damage via the Wnt/-catenin pathway. J Genet Genomics. 2021 OcRead more>>
VALIDATION IMAGES

A431 cell lysates probed with KRT18 (3C3) Monoclonal Antibody, unconjugated (bsm-33101M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: MCF-7 Cell lysates; Lane 2: HepG2 Cell lysates; probed with KRT18 (3C3) Monoclonal Antibody, unconjugated (bsm-33101M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation withKRT18 (3C3) Monoclonal Antibody (bsm-33101M) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Rat Small intestine lysates; Lane 2: Rat Pancreas lysates; Lane 3: Rat Lung lysates; Lane 4: Rat Uterus lysates probed with KRT18 Monoclonal Antibody, Unconjugated (bsm-33101M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.