bsm-33113M [Primary Antibody]
IL2RA/CD25 monoclonal antibody
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Host: Mouse

Target Protein: IL2RA/CD25

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3559

Swiss Prot: P01589

Source: KLH conjugated synthetic peptide derived from human IL2RA/CD25

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009].

Size: 100µL

Concentration: 1mg/ml

Applications: FCM(FCM(1ug/Test))

Predicted Molecular Weight: 28


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.


SR cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD25 .Antibody(bsm-33113M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).