bsm-33114M [Primary Antibody]
Histone H3 (di methyl K9) Monoclonal Antibody
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Host: Mouse

Target Protein: Histone H3(di methyl K9)

Modification Site: K9

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8290

Swiss Prot: P68431

Source: KLH conjugated synthetic peptide derived from human Histone H3(di methyl K9)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Histone H3(di methyl K9) monoclonal antibody, unconjugated (bsm-33114M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.