bsm-33116M [Primary Antibody]
Histone H3 (mono methyl K9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Histone H3 (mono methyl K9)

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: P68431

Swiss Prot: 8290

Source: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of mono methyl K9

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H3 (mono methyl K9) Monoclonal Antibody, Unconjugated (bsm-33116M) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human Siha cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human U-2OS cell lysates; Lane 5: Human Jurkat cell lysates probed with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33116M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human MOLT4 cell lysates; Lane 5: Human 293T cell lysates; Lane 6: Human U251 cell lysates probed with Histone H3 (mono methyl K9) Monoclonal Antibody, Unconjugated (bsm-33116M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.