bsm-33160M [Primary Antibody]
Actin, alpha skeletal muscle Monoclonal Antibody
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Host: Mouse

Target Protein: Actin, alpha skeletal muscle

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 58

Swiss Prot: P68133

Source: KLH conjugated synthetic peptide derived from human ACTA1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:500-1000))

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Actin, alpha skeletal muscle monoclonal antibody, unconjugated (bsm-33160M) at 1:50000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.