bsm-33167M [Primary Antibody]
Fumarase/FH (4C12) Monoclonal Antibody
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Host: Mouse

Target Protein: FH

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2271

Swiss Prot: P07954

Source: Recombinant human Fumarase/FH Protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]

Size: 100uL

Concentration: 1ug/ul

Applications: WB(WB=1:500-3000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fumarase/FH) Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded HumanSmall Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FH | Fumarase Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:1500 overnight at 4°C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.