bsm-33170M-Biotin [Conjugated Primary Antibody]
Vimentin Monoclonal Antibody, Biotin Conjugated
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DATASHEET

Host: Mouse

Target Protein: Vimentin

Specificity: KO-Validated

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7431

Swiss Prot: P08670

Source: Recombinant human Vimentin Protein

Purification: Purified by Protein G.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 Publication Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu H et al. Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 2020 Jun;70:109595.Read more>>
  • Juan-Juan Zhu. et al. VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation. Front Cell Dev Biol. 2020; 8: 576826Read more>>
  • Qi-Lin Lu. et al. Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy. MOL MED REP. 2022 Sep;26(3):1-9Read more>>
  • Sijie Wang. et al. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. CELL PROLIFERAT. 2022 Sep;:e13337Read more>>
  • Jianan Zhao. et al. DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting -alanine and carnosine homeostasis. THERANOSTICS. 2023; 13(13): 4392C4411Read more>>
  • Chen Si-Yu. et al. miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway. MOL BIOTECHNOL. 2024 Feb;:1-16Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.