DATASHEET
Host:
Mouse
Target Protein:
mStrawberry
Clonality:
Monoclonal
Isotype:
IgG
Source:
Recombinant Protein mStrawberry
Purification:
Purified by Protein G.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
VALIDATION IMAGES
Lane 1: mStrawberry-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33175P) probed with mStrawberry-Tag Monoclonal Antibody, Unconjugated (bsm-33175M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
mStrawberry tag was immunoprecipitated from mStrawberry expression vector whole cell lysate with bsm-33175M at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.
Lane 1: mStrawberry expression vector whole cell lysate (Input).
Lane 2: bsm-33175M IP in mStrawberry expression vector whole cell lysate.
Lane 3: native mouse IgG IP in mStrawberry expression vector whole cell lysate (negative control).