bsm-33185M [Primary Antibody]
EBFP (7B12) Monoclonal Antibody
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Host: Mouse

Target Protein: EBFP

Clonality: Monoclonal

Isotype: IgG

Source: Recombinant EBFP protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 26kDa


Cross Reactive Species: Species independent

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: EBFP-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33185P);Lane 2: Control (-) E.coli lysates probed with EBFP-Tag Monoclonal Antibody, Unconjugated (bsm-33185M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


EBFP tag was immunoprecipitated from EBFP expression vector whole cell lysate with bsm-33185M at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody. Lane 1: EBFP expression vector whole cell lysate (Input). Lane 2: bsm-33185M IP in EBFP expression vector whole cell lysate. Lane 3: native mouse IgG IP in EBFP expression vector whole cell lysate (negative control).