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Host: Rabbit
Target Protein: alpha smooth muscle Actin
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 59
Swiss Prot: P62736
Source: KLH conjugated synthetic peptide derived from human Actin alpha
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications: WB(WB(1:1000-20000))
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Chicken
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with alpha smooth muscle Actin monoclonal antibody, unconjugated (bsm-33187R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with alpha smooth muscle Actin monoclonal antibody, unconjugated (bsm-33187R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.