bsm-33189M [Primary Antibody]
ECFP-Tag (10H5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Enhanced Cyan Fluorescent Protein

Clonality: Monoclonal

Isotype: IgG

Source: Recombinant ECFP

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 27kDa


Cross Reactive Species: Others

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: ECFP-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33189P) probed with ECFP-Tag Monoclonal Antibody, Unconjugated (bsm-33189M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.