bsm-33190M-BF555 [Conjugated Primary Antibody]
Caspase 8 Monoclonal Antibody, AbBy Fluor® 555 Conjugated
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Caspase 8

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 841

Swiss Prot: Q14790

Source: Recombinant human Caspase 8 Protein

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Conjugation: AbBy Fluor® 555

Excitation/ Emission: 553nm/568nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu B et al. Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anteriorcruciate ligament-transected rat osteoarthritis model. Life Sci. 2019 Jul 1;228:158-166. Read more>>
  • Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
  • Zhao W et al. Junduqing extractive promotes the apoptosis of nasopharyngeal carcinoma cells through down-regulating Mcl-1 and Bcl-xL and up-regulating Caspase-3, Caspase-8 and Caspase-9. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3904-3912.Read more>>
  • Lei Xiang. et al. Antitumor effects of curcumin on the proliferation, migration and apoptosis of human colorectal carcinoma HCT?116 cells. Oncol Rep. 2020 Nov;44(5):1997-2008Read more>>
  • Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-_B pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166Read more>>
  • Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 89824Read more>>
  • Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28Read more>>
  • Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539Read more>>
  • Hai-bo Liu. et al. Dispelling Dampness, Relieving Turbidity and Dredging Collaterals Decoction, Attenuates Potassium Oxonate-Induced Hyperuricemia in Rat Models. DRUG DES DEV THER. 2023 Aug 01Read more>>
  • Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
  • Shuli Yang. et al. Enrichment and Evaluation of Antitumor Properties of Total Flavonoids from Juglans mandshurica Maxim. MOLECULES. 2024 Jan;29(9):1976Read more>>
  • o Fang. et al. Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model. BIOMOLECULES. 2024 Aug;14(8):901Read more>>
VALIDATION IMAGES