bsm-33193M-A680 [Conjugated Primary Antibody]
Cytochrome C (6B10) Monoclonal Antibody, ALEXA FLUOR® 680 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Cytochrome C

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 54205

Swiss Prot: P99999

Source: Recombinant human Cytochrome C Protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Conjugation: ALEXA FLUOR® 680

Excitation/ Emission: 679nm/702nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB()
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ding, Wensen, et al. "Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage." Brain Research Bulletin 128 (2017): 40-47.Read more>>
  • Zhou BZ et al. Protective effect of cyclosporine A in the treatment of severe hydronephrosis in a rabbit renal pelvic perfusion model. Turk J Med Sci. 2019 Oct 24;49(5):1590-1598.Read more>>
  • Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435Read more>>
  • Xuena Zhang. et al. Luteoloside Prevents Sevoflurane-induced Cognitive Dysfunction in Aged Rats via Maintaining Mitochondrial Function and Dynamics in Hippocampal Neurons. NEUROSCIENCE. 2023 FebRead more>>
  • Yanxin Jia. et al. Wnt10b knockdown promotes UCP1 expression in brown adipose tissue in mice. GENES CELLS. 2023 SepRead more>>
VALIDATION IMAGES