bsm-33199M [Primary Antibody]
Caspase 3 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: Caspase 3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 836

Swiss Prot: P42574

Source: Recombinant human Active Caspase 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Peng et al. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells. (2018) Oncol.Lett. 16:785-792Read more>>
  • Ou X et al. Desumoylating Isopeptidase 2 (DESI2) Inhibits Proliferation and Promotes Apoptosis of Pancreatic Cancer Cells through Regulating PI3K/AKT/mTOR Signaling Pathway. (2018)Pathol. Oncol. Res. Nov 8.Read more>>
  • Sha J et al. Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor. Toxicol Appl Pharmacol. 2019 Feb 1;364:144-152. Read more>>
  • Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
  • Hong Cheet al. circ_0080145 Enhances Imatinib Resistance of Chronic Myeloid Leukemia by Regulating miR-326/PPFIA1 Axis. Cancer Biother Radiopharm. 2020 Jun 27.Read more>>
  • Hongwei Duan. et al. Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development. J Steroid Biochem. 2021 Mar;207:105819Read more>>
  • Xing Xue. et al. Effects of local dexmedetomidine administration on the neurotoxicity of ropivacaine for sciatic nerve block in rats. Mol Med Rep. 2020 Nov;22(5):4360-4366Read more>>
  • Ying Feng. et al. LncRNA MALAT1 inhibits apoptosis of endometrial stromal cells through miR-126-5p-CREB1 axis by activating PI3K-AKT pathway. Mol Cell Biochem. 2020 Dec;475(1):185-194Read more>>
  • Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:11235Read more>>
  • Mingying Han. et al. Verapamil inhibits early acute liver failure through suppressing the NLRP3 inflammasome pathway. 2021 May 24Read more>>
  • Xuanxuan Jiang. et al. Arsenic (III) and/or Antimony (III) induced disruption of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in mice heart. Ecotox Environ Safe. 2021 Sep;220:112394Read more>>
  • Qiu Lei. et al. MiR-526b-3p Inhibits the Resistance of Glioma Cells to Adriamycin by Targeting MAPRE1. J Oncol. 2022;2022:2402212Read more>>
  • Suihui Li. et al. Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1. Histol Histopathol. 2022 Feb 21;1844Read more>>
  • Xiangyou Yu. et al. Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3_-dependent manner. TISSUE CELL. 2022 Jun;76:101813Read more>>
  • Eman M. Awad. et al. Dihydromyricetin protects against high glucose-induced endothelial dysfunction: Role of HIF-1_/ROR2/NF-_B. BIOMED PHARMACOTHER. 2022 Sep;153:113308Read more>>
  • Qin, Qiaozhen. et al. Ectoderm-derived frontal bone mesenchymal stem cells promote traumatic brain injury recovery by alleviating neuroinflammation and glutamate excitotoxicity partially via FGF1. STEM CELL RES THER. 2022 Dec;13(1):1-19Read more>>
  • Jingyan Li. et al. Uncovering the Effect and Mechanism of Rhizoma Corydalis on Myocardial Infarction Through an Integrated Network Pharmacology Approach and Experimental Verification. FRONT PHARMACOL. 2022; 13: 927488Read more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • Xing, Jing. et al. CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via the miR-140-5p/MLKL axis. J BIOENERG BIOMEMBR. 2022 Aug;:1-12Read more>>
  • Rui Guo. et al. Ulinastatin attenuates spinal cord injury by targeting AMPK/NLRP3 signaling pathway. J CHEM NEUROANAT. 2022 Nov;125:102145Read more>>
  • Haiyang Zhang. et al. Melatonin attenuates chronic stress-induced hippocampal inflammatory response and apoptosis by inhibiting ADAM17/TNF- axis. FOOD CHEM TOXICOL. 2022 Sep;:113441Read more>>
  • Jing Wang. et al. The inhibitory effect of Yam polysaccharides on acrylamide-induced programmed cell death in RAW 264.7 cells. FOOD SCI NUTR. 2022 SepRead more>>
  • Refaie, Marwa M. M.. et al. Dapagliflozin Guards Against Cadmium-Induced Cardiotoxicity via Modulation of IL6/STAT3 and TLR2/TNF Signaling Pathways. CARDIOVASC TOXICOL. 2022 Oct;:1-13Read more>>
  • Xijuan Wang. et al. Caffeine reduces oxidative stress to protect against hyperoxia-induced lung injury via the adenosine A2A receptor/cAMP/PKA/Src/ERK1/2/p38MAPK pathway. REDOX REP. 2022 Nov 1Read more>>
  • Yuanchao Ling. et al. Bisphenol A exposure induces testicular oxidative damage via FTO/m6A/Nrf2 axis during postnatal development in mice. J APPL TOXICOL. 2022 NovRead more>>
  • Ying Yang. et al. Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3 to regulate macrophage/microglial polarization. PHYTOMEDICINE. 2022 Dec;:154602Read more>>
  • Junzhao Yuan. et al. Rat Hepatocytes Mitigate Cadmium Toxicity by Forming Annular Gap Junctions and Degrading Them via Endosome–Lysosome Pathway. INT J MOL SCI. 2022 Jan;23(24):15607Read more>>
  • Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436Read more>>
  • Wang Mingming. et al. Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells. J CANCER. 2023 Feb;14(4):611-627Read more>>
  • Wen Jiang. et al. Hirsutine ameliorates myocardial ischemia-reperfusion injury through improving mitochondrial function via CaMKII pathway. CLIN EXP HYPERTENS. 2023;45(1):Article: 2192444Read more>>
  • Marwa Monier Mahmoud Refaie. et al. Molecular mechanisms mediate roflumilast protective effect against isoprenaline-induced myocardial injury. IMMUNOPHARM IMMUNOT. 2023 Jun 19Read more>>
  • Xishuai Tong. et al. Vitamin D Alleviates Cadmium-Induced Inhibition of Chicken Bone Marrow Stromal Cells Osteogenic Differentiation In Vitro. ANIMALS. 2023 Jan;13(15):2544Read more>>
  • Liu Xueqi. et al. In Vivo Evaluation of Self-assembled nano-Saikosaponin-a for Epilepsy Treatment. MOL BIOTECHNOL. 2023 Aug;:1-11Read more>>
  • lanca Ocampo-Garca. et al. 225Ac-iPSMA-RGD for Alpha-Therapy Dual Targeting of Stromal/Tumor Cell PSMA and Integrins. INT J MOL SCI. 2023 Jan;24(23):16553Read more>>
  • Xu Zhenhua. et al. Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression. CELL DEATH DIS. 2024 Feb;15(2):1-16Read more>>
  • Qingsha Hou. et al. ATP5me alleviates high glucose-induced myocardial cell injury. INT IMMUNOPHARMACOL. 2024 Mar;129:111626Read more>>
  • Di Zhao. et al. Protective effect of exosomes derived from bone marrow mesenchymal stem cells on hypoxia reperfusion injury of cardiomyocytes. CELL MOL BIOL. 2024 Feb;70(2):73-8Read more>>
VALIDATION IMAGES

HL60 lysates probed with Active Caspase 3 Polyclonal Antibody, Unconjugated (bs-33199M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Active Caspase 3 Monoclonal Antibody, Unconjugated (bsm-33199M) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Active Caspase 3) Monoclonal Antibody, Unconjugated (bsm-33199M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.


Lane 1: Recombinant human Caspase-3 protein, His & MBP (bs-41233P) probed with Active Caspase 3 Monoclonal Antibody, Unconjugated (bsm-33199M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.