support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Mouse
Target Protein: Acetyl Lysine
Clonality: Monoclonal
Isotype: IgG
Source: BSA conjugated Acetylated Lysine
Purification: Purified by Protein G.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
FCM(FCM(1μg/Test))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IF(ICC)(IF(ICC)(1:50-200))
Cross Reactive Species: Species independent
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
The Hela (Treated Sodium Butyrate (5mM, 24h))(H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Acetyl Lysine antibody (bsm-33202M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela（Treated Sodium Butyrate (5mM, 24h)） (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl Lysine) monoclonal Antibody, unconjugated (bsm-33202M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.