bsm-33219M [Primary Antibody]
PI3K p85 (5C11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: PI3K p85

Immunogen Range: 1-110/724


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 18708

Swiss Prot: P26450

Source: Recombinant mouse PI3K p85 Protein

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang K et al. Neat1-miRNA204-5p-PI3K-AKT axis as a potential mechanism for Photodynamic Therapy treated Colitis in Mice. (2018) Photodiagnosis Photodyn Ther. Oct 29. Read more>>
  • Qamar H et al. Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling. Biomolecules. 2019 Nov 26;9(12). Read more>>
  • Huang Xiao-shuang. et al. Mechanism of Action of Acupotomy in Inhibiting Chondrocyte Apoptosis in Rabbits with KOA through the PI3K/Akt Signaling Pathway. Evid-Based Compl Alt. 2020;2020:4241917Read more>>
  • Luo Yan. et al. Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis. EVID-BASED COMPL ALT. 2022;2022:4194827Read more>>
  • Tian Wang. et al. HMGA1 As a Potential Prognostic and Therapeutic Biomarker in Breast Cancer. DIS MARKERS. 2022 Nov 26;2022:7466555Read more>>
  • Xu, Qinqin. et al. Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice. MOL MED. 2022 Dec;28(1):1-14Read more>>
VALIDATION IMAGES

BRL-3A cell lysates probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: NIH/3T3 Cell lysates; Lane 2: RAW246.7 Cell lysates; probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Human Jurkatcell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Heart lysates; Lane 6: Rat Heart lysates; Lane 7: Mouse Skeletal muscle lysates; Lane 8: Rat Skeletal muscle lysates; Lane 9: Mouse Spleen lysates; Lane 10: Human MOLT-4 cell lysates probed with PI3K p85 Monoclonal Antibody, Unconjugated (bsm-33219M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.